Journal: Journal of neuropathology and experimental neurology

Article Title: A role for interferon-gamma in focal cerebral ischemia in mice.

doi: 10.1093/jnen/63.9.942

Figure Lengend Snippet: Fig. 4. Immunohistochemical staining for the cell adhesion molecule ICAM-1/CD54 in (A, C) normal IFNg-Tg and (B, D) normal SJL mice showing similar endothelial (arrows in A and B) and microglial ICAM-1 expression in IFNg-Tg and SJL mice. High magnification of ICAM-1-immunoreactive vessels (arrows in C and D) and microglial-like cells (arrowheads in C and D) in IFNg-Tg (C) and SJL (D) mice. Scale bars: (A,B) 5 500 mm; (C,D) 5 30 mm.

Article Snippet: The primary antibody was a rat monoclonal antibody to murine ICAM-1 (CD54) (MCA818, Serotec) (1:200).

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: eLife

Article Title: Cellular entry and uncoating of naked and quasi-enveloped human hepatoviruses

doi: 10.7554/eLife.43983

Figure Lengend Snippet:

Article Snippet: Antibody (sheep) , anti-ICAM-1 , R and D Systems , AF720 , (WB) 1:250.

Techniques: Virus

Journal: EMBO Reports

Article Title: T cell‐dependent bispecific antibodies alter organ‐specific endothelial cell–T cell interaction

doi: 10.15252/embr.202255532

Figure Lengend Snippet:

Article Snippet: HUVECs transfected with either negative control siRNA or siRNA specific for CD9 were incubated either in control medium or with TDB‐conditioned PBMC supernatant with and without 12.5 μg/ml anti‐ICAM‐1 (R&D systems, ref: AF720) for 24 h at 37°C, 5% CO 2 .

Techniques: Recombinant, Purification, Blocking Assay, Produced, RNAscope, Sequencing, Negative Control, Protease Inhibitor, Software, Multiplex Assay, Cell Isolation, Enzyme-linked Immunosorbent Assay, Library Quantification, BIA-KA, Gene Expression

Journal:

Article Title: Neutrophil String Formation: Hydrodynamic Thresholding and Cellular Deformation during Cell Collisions

doi: 10.1529/biophysj.103.035782

Figure Lengend Snippet: Neutrophil-neutrophil interactions during string formation. Individual cell-cell interactions were visualized using videomicroscopy. A free-flowing neutrophil (open circle) collides with a preadhered neutrophil (dashed circle). The primary collision efficiency (ɛ) is the ratio of collisions with bonding to the total number of collisions. The flowing cell may either release from the primary cell or form a secondary adhesive event with the ICAM-1 or fibrinogen coated on the surface of the chamber. X2 refers to the position, measured from the outer edge of the primary cell to the center of the flowing cell, where the flowing cell makes its first adhesive interaction with the surface. This rolling cell may then release from this position into the free-flowing stream, or may reattach further downstream from its initial point of interaction.

Article Snippet: Recombinant human ICAM-1 (rhICAM-1) immunoglobulin G (IgG) chimera was purchased from R&D Systems (Minneapolis, MN) and diluted with Hanks' buffered saline (HBS).

Techniques: Adhesive

Journal:

Article Title: Neutrophil String Formation: Hydrodynamic Thresholding and Cellular Deformation during Cell Collisions

doi: 10.1529/biophysj.103.035782

Figure Lengend Snippet: Primary collision efficiencies (ɛ) and interaction times for neutrophil collisions. Neutrophils were perfused at different shear rates over neutrophils adherent to ICAM-1 or fibrinogen for measurement of ɛ (A and B) and interaction times (C and D). Interaction times for collision with bonding (solid bar) or without detectable bonding (open bar) represent interaction times calculated for at least 30 interactions, with the exception of the fibrinogen at 300 s−1 for which only 17 interactions were observed.

Article Snippet: Recombinant human ICAM-1 (rhICAM-1) immunoglobulin G (IgG) chimera was purchased from R&D Systems (Minneapolis, MN) and diluted with Hanks' buffered saline (HBS).

Techniques: Shear

Journal:

Article Title: Neutrophil String Formation: Hydrodynamic Thresholding and Cellular Deformation during Cell Collisions

doi: 10.1529/biophysj.103.035782

Figure Lengend Snippet: Primary collision efficiency for neutrophils treated with various monoclonal antibodies. Neutrophils were incubated with 20 μg/ml mAb against known surface receptors for 20 min at room temperature. The treated neutrophils were then perfused over nontreated, ICAM-1 adherent neutrophils. In the case of treatment with anti-L-selectin, both ICAM-1 adherent and perfused neutrophils were treated with mAb. The primary collision efficiency was calculated for neutrophils treated with each of the antibodies tested.

Article Snippet: Recombinant human ICAM-1 (rhICAM-1) immunoglobulin G (IgG) chimera was purchased from R&D Systems (Minneapolis, MN) and diluted with Hanks' buffered saline (HBS).

Techniques: Incubation

Journal:

Article Title: Neutrophil String Formation: Hydrodynamic Thresholding and Cellular Deformation during Cell Collisions

doi: 10.1529/biophysj.103.035782

Figure Lengend Snippet: Neutrophils deform during primary collision at 100 s−1. Neutrophils were perfused at 100 s−1 over neutrophils preadhered to an ICAM-1 surface, and primary adhesive interactions were imaged using a high speed digital camera at 240 fps.

Article Snippet: Recombinant human ICAM-1 (rhICAM-1) immunoglobulin G (IgG) chimera was purchased from R&D Systems (Minneapolis, MN) and diluted with Hanks' buffered saline (HBS).

Techniques: Adhesive

Journal:

Article Title: Neutrophil String Formation: Hydrodynamic Thresholding and Cellular Deformation during Cell Collisions

doi: 10.1529/biophysj.103.035782

Figure Lengend Snippet: Strings of neutrophils form on ICAM-1 but not fibrinogen-coated surfaces. Fluo-4 treated neutrophils were perfused at various shear rates over ICAM-1 adherent (top) or fibrinogen-adherent (bottom) neutrophils. Cells formed linear string-like structures on preseeded ICAM-1 surfaces at shears between 60 and 200 s−1, with maximal string formation occurring at 100 s−1. Strings failed to form on fibrinogen-coated surfaces for any of the shear stresses tested.

Article Snippet: Recombinant human ICAM-1 (rhICAM-1) immunoglobulin G (IgG) chimera was purchased from R&D Systems (Minneapolis, MN) and diluted with Hanks' buffered saline (HBS).

Techniques: Shear

Journal:

Article Title: Neutrophil String Formation: Hydrodynamic Thresholding and Cellular Deformation during Cell Collisions

doi: 10.1529/biophysj.103.035782

Figure Lengend Snippet: Secondary adhesion for neutrophils on ICAM-1 and fibrinogen have very different lifetimes. Neutrophils were perfused at shear rates of 10–300 s−1 over ICAM-1 or fibrinogen-adherent cells, and the adhesive fraction of the cells was determined. Interactions were binned based upon the duration of the adhesive event: highly transient (0–500 ms), transient (500–3500 ms), and firmly adherent (≥3500 ms). The contribution of each interaction type to the overall adhesive fraction was calculated for all shear rates tested.

Article Snippet: Recombinant human ICAM-1 (rhICAM-1) immunoglobulin G (IgG) chimera was purchased from R&D Systems (Minneapolis, MN) and diluted with Hanks' buffered saline (HBS).

Techniques: Shear, Adhesive

Journal:

Article Title: Neutrophil String Formation: Hydrodynamic Thresholding and Cellular Deformation during Cell Collisions

doi: 10.1529/biophysj.103.035782

Figure Lengend Snippet: Activation profiles for neutrophils in ICAM-1 during string formation. Activation of neutrophils as they formed strings on an ICAM-1 coated surface was monitored by loading the cells with Fluo-4 calcium indicator. The fluorescence of the neutrophils is directly proportional to the activation state of the cell. A trace of fluorescence intensity versus time was generated for adhering cells in the string of neutrophils.

Article Snippet: Recombinant human ICAM-1 (rhICAM-1) immunoglobulin G (IgG) chimera was purchased from R&D Systems (Minneapolis, MN) and diluted with Hanks' buffered saline (HBS).

Techniques: Activation Assay, Fluorescence, Generated

Journal:

Article Title: Neutrophil String Formation: Hydrodynamic Thresholding and Cellular Deformation during Cell Collisions

doi: 10.1529/biophysj.103.035782

Figure Lengend Snippet: Activation profiles for shear-induced neutrophil activation. Activation of neutrophils was monitored through fluorescence via Fluo-4 calcium indicator. Cells were loaded with the dye and then perfused into the ICAM-1-coated chamber, at which point the flow was turned off. The cells were monitored for 3 min in static environment during cell adhesion to the surface, and then the fluorescent lamp was turned off to avoid photobleaching. The lamp was turned on again after 9 min, and then flow at 200 s−1 was restarted at t = 10 min. Activation of the cells was monitored by fluorescence again from 9 to 12 min. Three representative responses from individual cells are shown in A–C.

Article Snippet: Recombinant human ICAM-1 (rhICAM-1) immunoglobulin G (IgG) chimera was purchased from R&D Systems (Minneapolis, MN) and diluted with Hanks' buffered saline (HBS).

Techniques: Activation Assay, Shear, Fluorescence